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Human TBCA Antibody

Catalog Number:

ATGA0155

Size (100ul)$ 290 USD
Size (50ul)$ 170 USD
Quantity

Datasheet

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Product Information

Catalog Number:

ATGA0155

Clone:

AT1A5

Product type:

Monoclonal Antibody

UniProt No.:

O75347

NCBI Accession No.:

NP_004598

Alternative Name:

Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a

Product Specification

Antibody Host:

Mouse

Reacts with:

Human

Concentration:

1mg/ml

Formulation:

Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol

Immunogen:

Recombinant TBCA (1-108aa) purified from E. coli

Isotype:

IgG2b kappa

Purification Note:

By protein-G affinity chromatography

Application:

ELISA,WB,ICC/IF,FACS

Usage:

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage:

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis:

 

The cell lysate of LNCap(20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

 

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate

Immunofluorescence:

 

ICC/IF analysis of TBCA in A549 cells. The cell was stained with ATGA0155 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

 

ICC/IF analysis of TBCA in HeLa cells. The cell was stained with ATGA0155 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Flow Cytometry:

 

Flow cytometry analysis of TBCA in HeLa cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.


Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

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