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A549 Cell Lysate

Catalog Number:

ATGL0008

Size (500ug)$ 200 USD
Size (200ug)$ 110 USD
Size (100ug)$ 80 USD
Quantity

Datasheet

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Product Information

Catalog Number:

ATGL0008

Product type:

Cell Lysate

Product Specification

Concentration:

2mg/ml (determined by BCA assay)

Formulation:

Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage:

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Preparation before usage:

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

General Description:

A549 cells are adenocarcinomic human alveolar basal epithelial cells. The A549 cell line was initiated from the removal and culturing of cancerous lung tissue in the explanted tumor from 58-year-old male, in 1972. A549 cells is able to synthesize lecithin and contain high level of unsaturated fatty acids, which are important to maintain the membrane phospholipids in cells. A549 cell line are widely used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism and as a transfection host.

Quality Control:

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis:

 

All lanes : A549 Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)


Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

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