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G-361 Cell Lysate

Catalog Number:

ATGL0012

Size (500ug)$ 200 USD
Size (200ug)$ 110 USD
Size (100ug)$ 80 USD
Quantity

Datasheet

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Product Information

Catalog Number:

ATGL0012

Product type:

Cell Lysate

Product Specification

Concentration:

2mg/ml (determined by BCA assay)

Formulation:

Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage:

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Preparation before usage:

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

General Description:

G-361 cells are established from a malignant melanoma of a 31 year-old male. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR) -PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Quality Control:

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis:

 

All lanes : G-361 Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)


Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

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