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K562 Cell Lysate

Catalog Number:

ATGL0029

Size (500ug)$ 200 USD
Size (200ug)$ 110 USD
Size (100ug)$ 80 USD
Quantity

Datasheet

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Product Information

Catalog Number:

ATGL0029

Product type:

Cell Lysate

Product Specification

Concentration:

2mg/ml (determined by BCA assay)

Formulation:

Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage:

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Preparation before usage:

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

General Description:

K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

Quality Control:

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis:

 

All lanes : K562 Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)


Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

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