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C3H/101/2 Cell Lysate

Catalog Number:

ATGL0022

Size (500ug)$ 200 USD
Size (200ug)$ 110 USD
Size (100ug)$ 80 USD
Quantity

Datasheet

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Product Information

Catalog Number:

ATGL0022

Product type:

Cell Lysate

Product Specification

Concentration:

2mg/ml (determined by BCA assay)

Formulation:

Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage:

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Preparation before usage:

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

General Description:

C3H/10T1/2 cell line was isolated in 1972 from a line of C3H mouse embryo cells. The cells are contact sensitive and there is no detectable background spontaneous transformation. They are highly susceptible to transformation by chemical agents and express H-2k antigen. Tested and found negative for ectromelia virus.

Quality Control:

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Data

Western blot analysis:

 

All lanes : C3H/10T1/2 Whole Cell Lysate (40ug)
Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)


Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.

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