NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT19E8

UniProt No.

Q9UNE0

NCBI Accession No.

NP_071731

Alternative names

Tumor necrosis factor receptor superfamily member EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human EDAR (27-448aa) purified from E.coli

Isotype

IgG2a kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, FACS

Usage

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EDAR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant protein EDAR
Lane 2 : A549 cell lysate
Lane 3 : Ramos cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of EDAR in HeLa cells. The cell was stained with ATGA0334 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)