Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 47-574aa
- UniProt No.
- P30613
- NCBI Accession No.
- NP_000289
- Alternative names
- Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK
Product Specification
- Molecular Weight
- 59.2 kDa (549aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity: > 80unit/mg. One unit will convert 1.0 umole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.
- Tag
- His-Tag
- Applications
- Enzyme Activity,SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.