Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-522aa
- UniProt No.
- Q8BGT5
- NCBI Accession No.
- NP_776291
- Alternative names
- Alanine aminotransferase 2, ALT2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamate pyruvate transaminase 2
Product Specification
- Molecular Weight
- 60.1 kDa (542aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 2mM DTT
- Purity
- > 85% by SDS-PAGE
- Bioactivity
- Specific activity is > 50unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.