Recombinant mouse IL-1Ra/IL1RN protein

Cat No.
ATGP3884

Size

QTY

List Price

10ug

USD 70


20ug

USD 110

50ug

USD 180

100ug

USD 190

250ug

USD 250

500ug

USD 430

1mg

USD 720

USD 0



Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
27-178aa
UniProt No.
P25085
NCBI Accession No.
NP_001034790
Alternative names
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RA
Additional Information
ATGP1516 has been replaced with a catalog number ATGP3884.

Product Specification

Molecular Weight
20 kDa (177aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60ng/ml with Mouse IL-1 alpha.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity

Mouse IL-1ra/IL-1F3 inhibits Mouse IL-1 alpha induced cell proliferation in the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 60 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.