Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 52-750aa
- UniProt No.
- P08473
- NCBI Accession No.
- NP_000893
- Alternative names
- MME, Neprilysin, CALLA, CD10, CMT2T, NEP, SCA43, SFE, Atriopeptidase, Common acute lymphocyticleukemia antigen, Enkephalinase, Neutral endopeptidase, Skin fibroblast elastase, Membrane metalloendopeptidase, EPN
Product Specification
- Molecular Weight
- 80.9 kDa (708aa)
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 0.1mM PMSF, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 5,000pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.