Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-108aa
- UniProt No.
- P62942
- NCBI Accession No.
- NP_463460.1
- Alternative names
- FKBP prolyl isomerase 1A, FKBP1, FK506 binding protein 1a 12kDa, PPIase FKBP1A, Rotamase, 12 kDa FKBP, FK506 binding protein 1, FK506 binding protein 1A 12kDa, Immunophilin FKBP12, FK506 binding protein T cell 12 kD, FK506 binding protein 12, FKBP 12, FKBP12 Exip3, FKBP12C, Peptidyl-prolyl cis-trans isomerase, PKCI2, PPIase, Protein kinase C inhibitor 2, calstabin 1, Rotamase
Product Specification
- Molecular Weight
- 14.1 kDa (128aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 1mM DTT, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 800nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.