Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 36-509aa
- UniProt No.
- P09622
- NCBI Accession No.
- NP_000099
- Alternative names
- Dihydrolipoyl dehydrogenase mitochondrial, DLDH, E3, GCSL, LAD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, DLD, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, Glycine cleavage system L protein, Glycine cleavage system protein L, Lipoamide reductase, Lipoyl dehydrogenase, PHE 3,
Product Specification
- Molecular Weight
- 54.4 kDa (511aa)
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 200unit/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.