Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-321aa
- UniProt No.
- P0A9P4
- NCBI Accession No.
- YP_003044110.1
- Alternative names
- Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, FAD/NAD(P)-binding, TRXR
Product Specification
- Molecular Weight
- 34.6 kDa (321aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.