Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-319aa
- UniProt No.
- P32322
- NCBI Accession No.
- NP_008838
- Alternative names
- Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3
Product Specification
- Molecular Weight
- 35.5 kDa (339aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 umole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25C.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.