Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-339aa
- UniProt No.
- O00757
- NCBI Accession No.
- NP_003828
- Alternative names
- Fructose-16-bisphosphatase isozyme 2, Fructose-1,6-bisphosphatase isozyme 2,
Product Specification
- Molecular Weight
- 39.0 kDa (362aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 1,500pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.
- Tag
- His-Tag
- Applications
- Enzyme Activity,SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.