Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-98aa
- UniProt No.
- P04080
- NCBI Accession No.
- NP_000091
- Alternative names
- Cystatin-B, Stefin B, PME, CST6
Product Specification
- Molecular Weight
- 13 kDa (118aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- Enzyme Activity,SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.