Recombinant human Caspase-3 protein

Cat No.
ATGP3874

Size

QTY

List Price

10ug

USD 235


50ug

USD 720

USD 0

Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
29-277aa
UniProt No.
P42574
NCBI Accession No.
NP_116786
Alternative names
CASP3, CPP32, CPP32B, SCA-1, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1

Product Specification

Molecular Weight
29.4 kDa (256aa)ns)
Concentration
0.5mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. 20mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 5,000pmol/min/ug. One unit will liberate 1pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE,Enzyme Activity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. ..

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.