Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- AT2H8
- UniProt No.
- P00533
- NCBI Accession No.
- NP_005219
- Alternative names
- ERBB, ERBB1, Epidermal growth factor receptor, HER1, PIG61, epidermal growth factor receptor,Urogastrone, Proto-oncogene c-ErbB-1, Oncogene ERBB, Cell proliferation inducing protein 61, Cellgrowth inhibiting protein 40, Avian erythroblastic leukemia viral (verbb) oncogene homolog
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human EGFR (424-605aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of EGFR in HeLa cells. The cell was stained with ATGA0541 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: A431 cell lysate
Lane 1.: A431 cell lysate
Related Publications
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Tran AV, et al. Targeted and controlled drug delivery by multifunctional mesoporous silica nanoparticles with internal fluorescent conjugates and external polydopamine and graphene oxide layers. (Acta Biomater. 2018)
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Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.