Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 190IIC311
- UniProt No.
- P43628
- NCBI Accession No.
- NP_055326
- Alternative names
- Killer cell immunoglobulin-like receptor 2DL3, KIR2DL3, CD158B2, KIRCL23, NKAT2, KIR2DL3 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 2, NKAT-2, NKAT2a, NKAT2b, p58 natural killer cell receptor clone CL-6, p58 NK receptor, p58.2 MHC class-I-specific NK re, Killer cell immunoglobulin-like receptor 2DL3, Killer cell immunoglobulin-like receptor 2DL3 CD158b, GL183, Killer Cell Immunoglobulin Like Receptor 2DL3, Killer inhibitory receptor CL2 3, KIR023GB, MHC Class I NK Cell Receptor, Natural Killer Associated Transcript 2, p58, p58 Natural Killer Cell Receptor Clone CL6, p58.2 MHC Class I Specific NK Receptor,
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human KIR2DL3 (19-161aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of KIR2DL3 in HeLa cells. The cell was stained with AKR3006 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: KIR2DL1 recombinant protein
Lane 2.: KIR2DL3 recombinant protein
Lane 3.: KIR2DS4 recombinant protein
Lane 1.: KIR2DL1 recombinant protein
Lane 2.: KIR2DL3 recombinant protein
Lane 3.: KIR2DS4 recombinant protein
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.