Human KIR2DL3/CD158b2 antibody

Cat No.
AKR3006

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
190IIC311
UniProt No.
P43628
NCBI Accession No.
NP_055326
Alternative names
Killer cell immunoglobulin-like receptor 2DL3, KIR2DL3, CD158B2, KIRCL23, NKAT2, KIR2DL3 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 2, NKAT-2, NKAT2a, NKAT2b, p58 natural killer cell receptor clone CL-6, p58 NK receptor, p58.2 MHC class-I-specific NK re, Killer cell immunoglobulin-like receptor 2DL3, Killer cell immunoglobulin-like receptor 2DL3 CD158b, GL183, Killer Cell Immunoglobulin Like Receptor 2DL3, Killer inhibitory receptor CL2 3, KIR023GB, MHC Class I NK Cell Receptor, Natural Killer Associated Transcript 2, p58, p58 Natural Killer Cell Receptor Clone CL6, p58.2 MHC Class I Specific NK Receptor,

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human KIR2DL3 (19-161aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of KIR2DL3 in HeLa cells. The cell was stained with AKR3006 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: KIR2DL1 recombinant protein
Lane 2.: KIR2DL3 recombinant protein
Lane 3.: KIR2DS4 recombinant protein

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.