Human Growth Hormone antibody

Cat No.
AGH0401

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
g3H5
UniProt No.
P01241
NCBI Accession No.
NP_000506
Alternative names
GH1, Growth Hormone, GH, GH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin, GHN, hGH-N

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human growth hormone (27-217aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,Neutralization
Usage
The antibody has been tested by ELISA, Western blot and Neutralization analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human hGH protein (21-217aa)
Lane 2.: Recombinant human hGH protein (27-202aa)

Additional Information

Neutralization:Measured by its ability to neutralize Growth hormone induced proliferation in Nb2-11 rat lymphoma cells. The neutralization ED50 is less than or equal to 2ug/ml in the presence of 2.5 ng/ml recombinant protein human Growth hormone.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.