Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- g3H5
- UniProt No.
- P01241
- NCBI Accession No.
- NP_000506
- Alternative names
- GH1, Growth Hormone, GH, GH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin, GHN, hGH-N
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human growth hormone (27-217aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, Neutralization
- Usage
- The antibody has been tested by ELISA, Western blot and Neutralization analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human hGH protein (21-217aa)
Lane 2.: Recombinant human hGH protein (27-202aa)
Lane 1.: Recombinant human hGH protein (21-217aa)
Lane 2.: Recombinant human hGH protein (27-202aa)
Additional Information
Neutralization:Measured by its ability to neutralize Growth hormone induced proliferation in Nb2-11 rat lymphoma cells. The neutralization ED50 is less than or equal to 2ug/ml in the presence of 2.5 ng/ml recombinant protein human Growth hormone.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.