Human NKp46 antibody

Cat No.
AKR0408

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
n1D9
UniProt No.
O76036
NCBI Accession No.
NP_004820
Alternative names
Natural cytotoxicity triggering receptor 1 isoform a, NCR1, LY94, NCR, NKp46 Extracellular Ig-like domain, Natural killer cell p46-related protein, hNKp46, NK-p46, NKp46, NK cell-activating receptor, Lymphocyte antigen 94 homolog, CD335 antigen, Natural cytotoxicity triggering receptor 1, Natural cytotoxicity triggering receptor 1 isoform a

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Nkp46 (22-255aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, FACS
Usage
The antibody has been tested by ELISA, Western blot and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. * Note: This product is routinely tested on peripheral blood mononuclear cells(PBMCs) or natural killer(Nk) cells. The histogram is an example of the flow cytometric analyses.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human NKp46 protein

Flow cytometry (FACS)

Flow cytometry analysis of NKp46 in PBMC. The cell was stained with AKR0408 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with AKR0408 at 2-5ug for 1x10^6 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.