Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- n1D9
- UniProt No.
- O76036
- NCBI Accession No.
- NP_004820
- Alternative names
- Natural cytotoxicity triggering receptor 1 isoform a, NCR1, LY94, NCR, NKp46 Extracellular Ig-like domain, Natural killer cell p46-related protein, hNKp46, NK-p46, NKp46, NK cell-activating receptor, Lymphocyte antigen 94 homolog, CD335 antigen, Natural cytotoxicity triggering receptor 1, Natural cytotoxicity triggering receptor 1 isoform a
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Nkp46 (22-255aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, FACS
- Usage
- The antibody has been tested by ELISA, Western blot and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. * Note: This product is routinely tested on peripheral blood mononuclear cells(PBMCs) or natural killer(Nk) cells. The histogram is an example of the flow cytometric analyses.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human NKp46 protein
Lane 1.: Recombinant human NKp46 protein
Flow cytometry (FACS)
Flow cytometry analysis of NKp46 in PBMC. The cell was stained with AKR0408 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with AKR0408 at 2-5ug for 1x10^6 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.