NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

n1D9

UniProt No.

O76036

NCBI Accession No.

NP_004820

Alternative names

NKp46 Extracellular Ig-like domain, NK-p46, NK cell-activating receptor, NCR1, NCR, Natural killer cell p46-related protein, Natural cytotoxicity triggering receptor 1 isoform a, Natural cytotoxicity triggering receptor 1, Lymphocyte antigen 94 homolog, Ly96, LY94, hNKp46, CD335 antigen

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Nkp46 (22-255aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, FACS

Usage

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human NKp46 protein

Flow cytometry (FACS)

Magnify

Flow cytometry analysis of NKp46 in PBMC. The cell was stained with AKR0408 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Magnify

Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with AKR0408 at 2-5ug for 1x10^6 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

Related Publications

Ascierto ML, et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. (J Transl Med. 2013)
Haberthur K, et al. NKG2D ligand expression in pediatric brain tumors. (Cancer Biol Ther. 2016)
Qu S, et al. Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model. (Int J Cancer. 2018)