Human alpha-Synuclein antibody

Cat No.
ANC0603

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
5C2
UniProt No.
P37840
NCBI Accession No.
NP_000336
Alternative names
SNCA, NACP, PARK1, alpha-Synuclein Delta-NAC, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, Alpha-synuclein, Alpha synuclein, Alpha-synuclein, isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, Non-A-beta component of alzheimers disease amyloid, precursor of, PARK 1, PARK 4, PARK4, Parkinson disease familial 1, PD 1, PD1, Snca synuclein, alpha (non A4 component of amyloid precursor), Synuclein alpha, Synuclein, alpha (non A4 component of amyloid precursor)

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human alpha-synuclein (61-95) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of alpha-Synuclein in HeLa cells. The cell was stained with ANC0603 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human alpha-synuclein protein
Lane 2.: Recombinant human beta-synuclein protein
Lane 3.: Recombinant human gamma-synuclein protein
The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein (61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human a-synuclein(1-60aa)
Lane 2.: Recombinant human a-synuclein(1-95aa)
Lane 3.: Recombinant human a-synuclein(61-140aa)
Lane 4.: Recombinant human a-synuclein(96-140aa)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.