Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 5C2
- UniProt No.
- P37840
- NCBI Accession No.
- NP_000336
- Alternative names
- SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human alpha-synuclein (61-95) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of alpha-Synuclein in HeLa cells. The cell was stained with ANC0603 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human alpha-synuclein protein
Lane 2.: Recombinant human beta-synuclein protein
Lane 3.: Recombinant human gamma-synuclein protein
Lane 1.: Recombinant human alpha-synuclein protein
Lane 2.: Recombinant human beta-synuclein protein
Lane 3.: Recombinant human gamma-synuclein protein
The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein (61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human a-synuclein(1-60aa)
Lane 2.: Recombinant human a-synuclein(1-95aa)
Lane 3.: Recombinant human a-synuclein(61-140aa)
Lane 4.: Recombinant human a-synuclein(96-140aa)
Lane 1.: Recombinant human a-synuclein(1-60aa)
Lane 2.: Recombinant human a-synuclein(1-95aa)
Lane 3.: Recombinant human a-synuclein(61-140aa)
Lane 4.: Recombinant human a-synuclein(96-140aa)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.