NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

5C2

UniProt No.

P37840

NCBI Accession No.

NP_000336

Alternative names

SNCA, NACP, PARK1, alpha-Synuclein Delta-NAC, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, Alpha-synuclein, Alpha synuclein, Alpha-synuclein, isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, Non-A-beta component of alzheimers disease amyloid, precursor of, PARK 1, PARK 4, PARK4, Parkinson disease familial 1, PD 1, PD1, Snca synuclein, alpha (non A4 component of amyloid precursor), Synuclein alpha, Synuclein, alpha (non A4 component of amyloid precursor)

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human alpha-synuclein (61-95) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of alpha-Synuclein in HeLa cells. The cell was stained with ANC0603 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human alpha-synuclein protein
Lane 2.: Recombinant human beta-synuclein protein
Lane 3.: Recombinant human gamma-synuclein protein

Magnify

The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with human anti-alpha-Synuclein (61-95aa) antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human a-synuclein(1-60aa)
Lane 2.: Recombinant human a-synuclein(1-95aa)
Lane 3.: Recombinant human a-synuclein(61-140aa)
Lane 4.: Recombinant human a-synuclein(96-140aa)