Human UCH-L1/PGP9.5 antibody

Cat No.
APG0714

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
3D9
UniProt No.
P09936
NCBI Accession No.
NP_004172
Alternative names
ubiquitin carboxyl-terminal esterase L1, uCHL1, PGP9.5, 1-223aa, uCH-L1, EC 3.4.19.12, ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy, Neuron cytoplasmic protein 9.5, Park 5, Parkinson Disease 5, PGP95, uCH L1, Protein gene product 9.5, ubiquitin C terminal esterase L1, ubiquitin C terminal hydrolase (neuron specific), ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase isozyme L1, ubiquitin thiolesterase, ubiquitin thiolesterase L1

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PGP9.5 (1-223aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,IHC
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PGP9.5 in U87MG cells. The cell was stained with APG0714 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain tissue lysate
Lane 2.: U87MG cell lysate

IHC Comment And Pic

Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.