Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 3A4
- UniProt No.
- P20339
- NCBI Accession No.
- NP_004153
- Alternative names
- RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Rab5a (1-215aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of Rab5a in U87MG cells. The cell was stained with ARA0803 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The tissue lysate of mouse brain (45ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Rab5a (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Related Publications
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Del Cid-Pellitero E, et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. (Front Syst Neurosci. 2017)
Winer H, et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. (Nat Commun. 2018)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.