Human Hexokinase 1/2 antibody

Cat No.
AHK0812

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
1A7
UniProt No.
P52789
NCBI Accession No.
NP_000180
Alternative names
HK2, Hexokinase 2, EC 2.7.1.1, Hexokinase type II, HK II, Muscle form hexokinase, Hexokinase-2, hexokinase 2,, HK1, Hexokinase 1, EC 2.7.1.1, Hexokinase type I, HK I, Brain form hexokinase, Hexokinase-1, BB404130, dea, Glycolytic enzyme, HEXOKIN, Hexokinase PI, Hexokinase type I, Hexokinase, tumor isozyme, Hexokinase-A, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HKI, HXK1, mHk1-s

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Hexokinase 1 recombinant protein
Lane 2.: Hexokinase 2 recombinant protein
Lane 3.: Hexokinase 3 recombinant protein
Lane 4.: Hexokinase 4 recombinant protein
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase 1/2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: K562 cell lysate
Lane 2.: Jurkat cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of Hexokinase 1/2 in Jurkat cells. The cell was stained with AHK0812 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.