Human PPP1R14A antibody

Cat No.
APP0832

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
4H10
UniProt No.
Q96A00
NCBI Accession No.
NP_150281
Alternative names
Protein phosphatase 1 regulatory (inhibitor) subunit 14A, CPI17, CPI-17, PPP1INL, Protein phosphatase 1, regulatory (inhibitor) subunit 14A, PPP1R14A, Protein phosphatase 1, regulatory (inhibitor) subunit 14A CPI 17, CPI 17 alpha, CPI 17alpha, CPI17, PKC potentiated inhibitory protein of PP1, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Regulatory subunit 14A

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PPP1R14A (1-147aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PPP1R14A in A549 cells. The cell was stained with APP0832 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPP1R14A antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : A549 cell lysate
Lane 2. : NIH-3T3 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.