Human Cathepsin L antibody

Cat No.
ACT0905

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT18F6
UniProt No.
P07711
NCBI Accession No.
NP_001244901
Alternative names
cathepsin L1 preproprotein, cathepsin L1, cathepsin L, CATL, CTSL, FLJ31037, Major excreted protein

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human CTSL (18-333aa) purified from E. coli.
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of CTSL in A549 cells. The cell was stained with ACT0905 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate
The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: CTSB recombinant protein
Lane 2.: CTSD recombinant protein
Lane 3.: CTSE recombinant protein
Lane 4.: CTSF recombinant protein
Lane 5.: CTSH recombinant protein
Lane 6.: CTSK recombinant protein
Lane 7.: CTSL recombinant protein
Lane 8.: CTSS recombinant protein
Lane 9.: CTSW recombinant protein
Lane 10.: CTSZ recombinant protein

Flow cytometry (FACS)

Flow cytometry analysis of CTSL in A549 cells. The cell was stained with ACT0905 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.