Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 2A5
- UniProt No.
- Q96B18
- NCBI Accession No.
- NP_659493
- Alternative names
- Thymus expressed gene 3-like, Dapper antagonist of beta-catenin homolog 3 (Xenopus laevis), Arginine rich region 1, MGC15476
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human DACT3 (89-202aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, IHC
- Usage
- The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DACT3 antibody (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain tissue lysate
Lane 1.: Mouse Brain tissue lysate
IHC Comment And Pic
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.