Human FLIP antibody

Cat No.
AFL0926

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT8B12
UniProt No.
O15519
NCBI Accession No.
NP_003870
Alternative names
CASP8 and FADD-like apoptosis regulator isoform 1, CFLAR, CASH, CASP8AP1, CLARP, MRIT, Caspase homolog, Caspase-eight-related protein, Casper, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FLAME-1, Inhibitor of FLICE, I-FLICE, MACH-related inducer of toxicity

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FLIP (1-480aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of FLIP in HeLa cells. The cell was stained with AFL0926 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FLIP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: K562 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.