Human 14-3-3 epsilon antibody

Cat No.
ATGA0124

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT4F8
UniProt No.
P62258
NCBI Accession No.
NP_006752
Alternative names
YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human14-3-3 epsilon (1-255aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: 293T cell lysate
Lane 4.: A549 cell lysate
Lane 5.: Mouse Brain Tissue lysate
The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 14-3-3 Zeta Recombinant Protein
Lane 2.: 14-3-3 Beta Recombinant Protein
Lane 3.: 14-3-3 Epsilon Recombinant Protein
Lane 4.: 14-3-3 Eta Recombinant Protein
Lane 5.: 14-3-3 Gamma Recombinant Protein
Lane 6.: 14-3-3 Sigma Recombinant Protein
Lane 7.: 14-3-3 Tau Recombinant Protein

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.