Human FABP7/B-FABP antibody

Cat No.
ATGA0131

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1D1
UniProt No.
O15540
NCBI Accession No.
NP_001437
Alternative names
Fatty acid binding protein 7, Fatty acid binding protein 7, FABP7, Fatty acid binding protein 7 B FABP, BFABP, BLBP, Brain lipid binding protein, DKFZp547J2313, FABP 7, FABPB, Fatty acid binding protein 7 brain, Fatty acid binding protein brain, OTTHuMP00000017119, Mammary derived growth inhibitor related, MRG

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FABP7 (1-132aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of FABP7 in U87MG cells. The cell was stained with ATGA0131 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with ATGA0131 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Testis tissue lysate
The tissue lysate of mouse brain (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.