Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1F5
- UniProt No.
- P30405
- NCBI Accession No.
- NP_005720
- Alternative names
- peptidylprolyl isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Mitochondrial cyclophilin, CyP-M, Rotamase F
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PPIF (30-207aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, IHC
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PPIF in HeLa cells. The cell was stained with ATGA0139 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: PC3 cell lysate
Lane 1.: 293T cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: MCF7 cell lysate
Lane 4.: PC3 cell lysate
IHC Comment And Pic
Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPIF (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.