NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT4C11

UniProt No.

O40947

NCBI Accession No.

AAB62657

Alternative names

Human herpesvirus 8 ORF73, Kaposi's sarcoma-associated herpesvirus

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human ORF73/HHV8 (122-329aa) purified from E. coli

Isotype

IgG2a kappa

Purification

By protein-G affinity chromatography

Applications

ELISA,WB,IHC

Usage

The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

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The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ORF73/HHV8 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate (40ug)
Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug)

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The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate (40ug)
Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug)

IHC Comment And Pic

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Paraffin embedded sections of Kaposi's sarcoma tissue were incubated with anti-ORF73/HHV8 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)