Human CRABP1 antibody

Cat No.
ATGA0162

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1A1
UniProt No.
P29762
NCBI Accession No.
NP_004369
Alternative names
Cellular retinoic acid binding protein 1, CRABP, CRABP-I, CRABPI, RBP5, Cellular retinoic acid binding protein 1 CRABP 1, CRABP I, RBP 5, Retinoic acid binding protein I cellular

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human CRABP1 (1-137aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of CRABP1 in Balb/3T3 cells. The cell was stained with ATGA0162 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The tissue lysate(30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Eye Tissue lysate
The cell lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: MCF7 cell lysate
The cell lysates(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRABP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: CRABP1 Transfected 293T cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.