Human FUS2 antibody

Cat No.
ATGA0164

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2F4
UniProt No.
Q93015
NCBI Accession No.
NP_036323
Alternative names
Protein fusion-2, FuS2, FuS-2, NAT6, FuS 2, N acetyltransferase 6, Protein fus 2, Protein fusion 2

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant FUS2 (1-308aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of human A549 cells stained with Hoechst 33342 (Blue) and monoclonal anti-human FUS2 antibody (1:500) with Texas Red (red).

Western blot analysis (WB)

The cell lysates(1ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FUS2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: FUS2 Transfected 293T cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of FUS2 in Balb/3T3 cells. The cell was stained with ATGA0164 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.