Human UBE2S antibody

Cat No.
ATGA0165

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2C12
UniProt No.
Q16763
NCBI Accession No.
NP_055316.2
Alternative names
ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2S AA409170, MGC101982, uBE2S, ubiquitin carrier protein S, ubiquitin protein ligase S, ubiquitin conjugating enzyme E2 24 kD, ubiquitin conjugating enzyme E2S

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human UBE2S (1-222aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).

Western blot analysis (WB)

The cell lysates(10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: UBE2S Transfected 293T cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of UBE2S in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.