Human AGXT antibody

Cat No.
ATGA0166

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT2T4
UniProt No.
P21549
NCBI Accession No.
NP_000021
Alternative names
Serpin B5, Protease inhibitor 5, SERPINB5, alanine-glyoxylate aminotransferase, AGXT1, PH1, AGT, SPT, AGT1, oxalosis I, primary hyperoxaluria type 1, L-alanine: glyoxylate aminotransferase 1, serine:pyruvate aminotransferase, glycolicaciduria

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant AGXT (330-392aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green).
ICC/IF analysis of AGXT in Hep3B cells. The cell was stained with ATGA0166 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AGXT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human AGXT protein

Flow cytometry (FACS)

Flow cytometry analysis of AGXT in HeLa cells. The cell was stained with ATGA0166 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.