Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT16E3
- UniProt No.
- Q9ULY5
- NCBI Accession No.
- NP_055173
- Alternative names
- CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human MINCLE (41-219aa) purified from E.coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, IHC, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
Immunofluorescence of human HeLa cells stained with monoclonal anti-human CLEC4E antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Western blot analysis (WB)
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : MINCLE transfected 293T cell lysate
Lane 1. : 293T cell lysate
Lane 2. : MINCLE transfected 293T cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of CLEC4E in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
IHC Comment And Pic
Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human CLEC4E antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Related Publications
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Seifert L, et al. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. (Nature. 2016)
Zhao X, et al. Activation of C-Type Lectin Receptor and (RIG)-I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages. (J Infect Dis. 2020)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.