Human Lactate Dehydrogenase A/LDHA antibody

Cat No.
ATGA0180

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1A4
UniProt No.
P00338
NCBI Accession No.
NP_005557
Alternative names
L-lactate dehydrogenase A chain, GSD11, LDH1, LDHM, PIG19, L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein, LDH muscle subunit, M LDH, L lactate dehydrogenase A chain, l7R2, Lactate dehydrogenase 1, A chain, Lactate dehydrogenase A, Lactate dehydrogenase A4, Lactate dehydrogenase M, LDH muscle subunit, OTTMuSP00000017774, Proliferation-inducing gene 19, Renal carcinoma antigen NY-REN-59

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human LDHA (1-332aa) purified from E.coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: 293T cell lysate
Lane 3.: A431 cell lysate
Lane 4.: HeLa cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.