Human beta-Tubulin antibody

Cat No.
ATGA0187

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT5B3
UniProt No.
Q9BVA1
NCBI Accession No.
NP_821080
Alternative names
bA506K6.1, DKFZp566F223, FLJ98847, MGC8685, Tubulin beta-2B chain, tubulin beta 2B

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human beta-Tubulin (1-445aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, IHC
Usage
The antibody has been tested by ELISA, Western blot and IHC analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate

IHC Comment And Pic

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human beta-Tubulin antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.