Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT5B3
- UniProt No.
- Q9BVA1
- NCBI Accession No.
- NP_821080
- Alternative names
- TUBB2B, Tubulin beta 2B class Iib, Tubulin beta 2b
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human beta-Tubulin (1-445aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, IHC
- Usage
- The antibody has been tested by ELISA, Western blot and IHC analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate
Lane 1.: Mouse brain tissue lysate
IHC Comment And Pic
Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human beta-Tubulin antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.