Human NQO-2 antibody

Cat No.
ATGA0192

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1E3
UniProt No.
P16083
NCBI Accession No.
AAH06096
Alternative names
Ribosyldihydronicotinamide dehydrogenase, DHQV, DIA6, QR2, Ribosyldihydronicotinamide dehydrogenase Ox2, EC 1.10.99.2, MGC94180, NAD(P)H dehydrogenase quinone 2, NAD(P)H menadione oxidoreductase 1 dioxin inducible 2, NAD(P)H menadione oxidoreductase 2 dioxin inducible, NMOR2, NQO 2, NRH dehydrogenase, NRH:quinone oxidoreductase 2, OTTHuMP00000015948, OTTHuMP00000015949, OTTHuMP00000015953, Ox 2, QR2, Quinone, Quinone reductase 2, Ribosyldihydronicotinamide dehydrogenase

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human NQO2 (1-231aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of NQO2 in A549 cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of NQO2 in HeLa cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: A549 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.