Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT1E3
- UniProt No.
- P16083
- NCBI Accession No.
- AAH06096
- Alternative names
- Ribosyldihydronicotinamide dehydrogenase, DHQV, DIA6, QR2, Ribosyldihydronicotinamide dehydrogenase Ox2, EC 1.10.99.2, MGC94180, NAD(P)H dehydrogenase quinone 2, NAD(P)H menadione oxidoreductase 1 dioxin inducible 2, NAD(P)H menadione oxidoreductase 2 dioxin inducible, NMOR2, NQO 2, NRH dehydrogenase, NRH:quinone oxidoreductase 2, OTTHuMP00000015948, OTTHuMP00000015949, OTTHuMP00000015953, Ox 2, QR2, Quinone, Quinone reductase 2, Ribosyldihydronicotinamide dehydrogenase
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human NQO2 (1-231aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunflorescense (ICC/IF)
ICC/IF analysis of NQO2 in A549 cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of NQO2 in HeLa cells. The cell was stained with ATGA0192 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: A549 cell lysate
Lane 1.: HeLa cell lysate
Lane 2.: K562 cell lysate
Lane 3.: A549 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.