Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT38E7
- UniProt No.
- P82663
- NCBI Accession No.
- NP_071942
- Alternative names
- 28S ribosomal protein S25 mitochondrial, 28S ribosomal protein S25, mitochondrial, mitochondrial ribosomal protein S25, MRP-S25, RPMS25
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human MRPS25 (1-173aa) purified from E. coli
- Isotype
- IgG2a kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA,WB
- Usage
- The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody (1:500 ~ 1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.