Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT2F8
- UniProt No.
- P01116
- NCBI Accession No.
- NP_203524
- Alternative names
- GTPase Kras, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, NS3, RASK2, GTPase Kras,K-RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS 1, NS 3
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human KRAS (1-186aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0350 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : HepG2 cell lysate
Lane 3. : Ramos cell lysate
Lane 4. : A549 cell lysate
Lane 5. : Balb/3T3 cell lysate
Lane 1. : HeLa cell lysate
Lane 2. : HepG2 cell lysate
Lane 3. : Ramos cell lysate
Lane 4. : A549 cell lysate
Lane 5. : Balb/3T3 cell lysate
The Recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : KRAS recombinant protein (ATGP2062)
Lane 2. : HRAS recombinant protein (ATGP0516)
Lane 3. : NRAS recombinant protein (ATGP0492)
Lane 1. : KRAS recombinant protein (ATGP2062)
Lane 2. : HRAS recombinant protein (ATGP0516)
Lane 3. : NRAS recombinant protein (ATGP0492)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.