Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT45E8
- UniProt No.
- Q14457
- NCBI Accession No.
- NP_003757
- Alternative names
- Beclin 1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, Beclin 1 autophagy related, ATG6, ATG6 autophagy related 6 homolog, VPS30
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human BECN (1-450aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant protein (10ng) and cell lysate(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human BECN1 protein
Lane 2.: Balb/3T3 cell lysate
Lane 1.: Recombinant human BECN1 protein
Lane 2.: Balb/3T3 cell lysate
The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BECN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: BECN1 Transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: BECN1 Transfected 293T cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of BECN1 in HeLa cells. The cell was stained with ATGA0355 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.