Human PA28 Activator alpha Subunit/PSME1 antibody

Cat No.
ATGA0360

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT12H3
UniProt No.
Q06323
NCBI Accession No.
NP_006254
Alternative names
Proteasome activator complex subunit 1, IFI5111, MGC8628, PA28A, PA28alpha, REGalpha

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PSME1 (1-249aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PSME1 in PC3 cells. The cell was stained with ATGA0360 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : LNCap cell lysate
Lane 3. : A549 cell lysate
Lane 4. : 293T cell lysate
Lane 5. : Jurkat cell lysate
Lane 6. : Raji cell lysate
Lane 7. : A431 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.