Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT6A10
- UniProt No.
- P30044
- NCBI Accession No.
- NP_036226
- Alternative names
- Alu corepressor 1, Antioxidant enzyme B166, AOEB166, Liver tissue 2D-page spot 71B, PLP, Peroxiredoxin V, Prx-V, Peroxisomal antioxidant enzyme, TPx type VI, Thioredoxin peroxidase PMP20, Thioredoxin-dependent peroxiredoxin 5, ACR1, AOEB166, PRXV, SBBI10
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Peroxiredoxin 5 (53-214aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of PRDX5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The Recombinant Human PRDX5 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant PRDX5 protein
Lane 2. : MCF-7 cell lysate
Lane 3. : A549 cell lysate
Lane 4. : HeLa cell lysate
Lane 5. : 293T cell lysate
Lane 6. : Jurkat cell lysate
Lane 1. : Recombinant PRDX5 protein
Lane 2. : MCF-7 cell lysate
Lane 3. : A549 cell lysate
Lane 4. : HeLa cell lysate
Lane 5. : 293T cell lysate
Lane 6. : Jurkat cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of PRDX5 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.