NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

AT1C3

UniProt No.

P26447

NCBI Accession No.

NP_002952

Alternative names

S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human S100A4 (1-101aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of S100A4 in A549 cells. The cell was stained with ATGA0384 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

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ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody

Western blot analysis (WB)

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A549 cell lysate
Lane 3. : NIH/3T3 cell lysate
Lane 4. : 3T3-L1 cell lysate