Human S100A4 antibody

Cat No.
ATGA0384

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT1C3
UniProt No.
P26447
NCBI Accession No.
NP_002952
Alternative names
S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human S100A4 (1-101aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of S100A4 in A549 cells. The cell was stained with ATGA0384 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody

Western blot analysis (WB)

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A549 cell lysate
Lane 3. : NIH/3T3 cell lysate
Lane 4. : 3T3-L1 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.