Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- AT5C11
- UniProt No.
- P24666
- NCBI Accession No.
- NP_009030
- Alternative names
- Acid phosphatase 1 soluble isoform b, Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase, Acid phosphatase 1, soluble isoform b Acid phosphatase 1 soluble, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase, Low molecular weight phosphotyrosine protein phosphatase, Acid phosphatase of erythrocyte, PAP2, Protein tyrosine phosphatase, PTPase, Purple acid phosphatase, Red cell acid phosphatase 1.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human ACP1 (1-158aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA,WB,ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of ACP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western blot analysis (WB)
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : Jurkat cell lysate
Lane 3. : TF-1 cell lysate
Lane 4. : NIH/3T3 cell lysate
Lane 5. : HepG2 cell lysate
Lane 1. : HeLa cell lysate
Lane 2. : Jurkat cell lysate
Lane 3. : TF-1 cell lysate
Lane 4. : NIH/3T3 cell lysate
Lane 5. : HepG2 cell lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.