Human GPI antibody

Cat No.
ATGA0390

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT22G2
UniProt No.
P06744
NCBI Accession No.
NP_000166
Alternative names
Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36, EC 5.3.1.9, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36, G6PI, Glucose-6-phosphate isomerase

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human GPI (1-558aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,FACS,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

ICC/IF analysis of GPI in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPI antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot analysis (WB)

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPI antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : HepG2 cell lysate
Lane 3. : A549 cell lysate
Lane 4. : Jurkat cell lysate
Lane 5. : U87MG cell lysate
Lane 6. : Raji cell lysate
Lane 7. : MCF7 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of GPI in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.