Human NDRG1 antibody

Cat No.
ATGA0401

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
AT11G4
UniProt No.
Q92597
NCBI Accession No.
NP_006087
Alternative names
N-myc downstream regulated 1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated 1 TDD5, 42 kDa, anti-GC4, cmt4d, Differentiation related gene1 protein, Drg 1, hmsnl, Human mRNA for RTP complete cds, NDRG1, tdds, N myc downstream regulated gene 1, N myc downstream regulated gene 1 protein, Ndr 1, ndr1, NDRG 1, NDRG1 protein, Protein nickel specific induction protein, Nickel specific induction protein Cap43, nmsl, Nmyc downstream regulated, reduced in tumor, reducin, Nmyc downstream regulated gene1, Nmyc downstream regulated gene1 protein, Protein regulated by oxygen 1, Protein regulated by oxygen1, proxy1, Reducing agents and tunicamycin responsive protein, tunicamycin-responsive protein

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human NDRG1 (1-394aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of NDRG1 in HeLa cells. The cell was stained with ATGA0401 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NDRG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : LnCaP cell lysate
Lane 2. : HeLa cell lysate
Lane 3. : PC3 cell lysate
Lane 4. : Hep3B cell lysate
Lane 5. : HT1080 cell lysate
Lane 6. : Jurkat cell lysate
Lane 7. : SW480 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.